Device

Part:BBa_K249014:Design

Designed by: Roxanne Shank   Group: iGEM09_Lethbridge   (2009-08-26)

C-terminal YFP --> Tet inverter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Standard Prefix and Suffix, although there is BioFusion (aka Silver Lab) scar sequence fusing the YFP gene to the C-terminal Arginine Tag

Source

BioBrick Registry and synthesized Arganine Tag

References